NOTE: Design, construction, and performance standards are available from the NSF International (the National Sanitation Foundation), Ann Arbor, Michigan. Class II type B3 cabinets (also known as "convertible" cabinets) have all biologically contaminated internal ducts or plenums under negative pressure or surrounded by negative pressure ducts or plenums, exhaust HEPA filtered air through external ducts to space outside the laboratory, and have HEPA filtered downflow air that is a portion of the mixed downflow and inflow air from a common exhaust plenum. Class II type B2 cabinets (also know as "total exhaust" cabinets) have all biologically contaminated internal ducts or plenums under negative pressure or surrounded by negative pressure ducts or plenums, exhaust HEPA filtered air through external ducts to space outside the laboratory, and have HEPA filtered downflow air drawn from the laboratory or outside air. Class II type B1 cabinets have all biologically contaminated internal ducts or plenums under negative pressure or surrounded by negative pressure ducts or plenums, exhaust HEPA filtered air through external ducts to space outside the laboratory, and have HEPA filtered downflow air composed largely of unrecirculated inflow air. Class II type A cabinets may have positive pressure contaminated internal ducts and may exhaust HEPA filtered air into the laboratory. Class II biological safety cabinets are further classified as type A, type B1, type B2, and type B3. This cabinet provides HEPA-filtered downward air flow within the workspace. Exhaust air is filtered with a high efficiency particulate air filter (HEPA). The Class II vertical laminar flow biological safety cabinet is an open fronted, ventilated cabinet. This cabinet may be used in three operational modes with a full width open front, with an installed front closure panel not equipped with gloves, and with an installed front closure panel equipped with arm-length protective gloves.Ĭlass II. Exhaust air from the cabinet is filtered by a high efficiency particulate air (HEPA) filter and discharged without internal recirculation. The Class I biological safety cabinet is an open-fronted, negative pressure, ventilated cabinet. Three classes of biological safety cabinets are described below:Ĭlass I. A ventilated cabinet which serves as a primary containment device for operations involving biohazard agents or biohazardous materials. The publication states criteria for the determination of appropriate biosafety levels for microorganisms not listed. 93-8395, "Biosafety in Microbiological and Biomedical Laboratories", 1993. The National Institute of Health, Centers for Disease Control defines four levels of biosafety in HHS publication No. Biosafety levels consist of laboratory practices and techniques, safety equipment, and laboratory facilities appropriate for the operations performed and the hazard posed by the particular biohazard material. The agent includes the agent, products of infectious agents, or the components of infectious agents presenting a risk of illness or injury.īiohazardous materials are any materials that would harbor biohazardous agents such as human blood, body fluids, or tissues that may be contaminated with biohazardous agents. When biological safety cabinets, as defined below, are used to prevent harmful exposure from biohazard agents or biohazardous materials or hazardous substances they shall conform to the provisions of this Section and Section 5143.Įxception: Inspection doors or clean-out doors in exhaust ducts required by Section 5143(a)(3) do not apply to exhaust systems used in conjunction with biological safety cabinets.īiohazard agent means a replication capable pathogen which is a disease causing microorganism and is capable of causing diseases in humans including viruses, microbes and sub viral agents. Ventilation Requirements for Biological Safety Cabinets. Control of Hazardous SubstancesĪrticle 107.
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